Western blotting: evolution of an old analytical method to a new quantitative tool for biomarker measurements

Owen C, Fader KA & Hassanein M | Bioanalysis, 16(05), 319–328, (2024) Keywords: • antibodies • biomarker • protein detection • quantitative • validation • western blot Western blotting (WB) is a widely used laboratory technique for detecting specific proteins in biological matrices. Recent advances in antibody production, automation, gel and membrane manufacturing and highly sensitive detection platforms have transformed WB from a labor-intensive and qualitative method into a highly reproducible and quantitative assay suitable for biomarker detection. Despite these significant improvements in the capabilities and efficiency of WB, there remain challenges that hinder its widespread application as a research, diagnostic (in...