The Power of Selected Reaction Monitoring in Quantitative Proteomics

In this webinar, Dr Stephen Holman (Winner of the 2010 Bioanalysis Young Investigator Award) discussed the power of selected reaction monitoring (SRM) in quantitative proteomics. SRM has a long history of use in the area of quantitative mass spectrometry and in recent years the approach has seen increased application to quantitative proteomics, facilitating multiplexed relative and absolute quantification studies in a variety of organisms.

The application of SRM to quantitative proteomics was discussed along with the theory and advantages of SRM, the selection of peptide surrogates for protein quantification, the design of optimal SRM co-ordinates and the handling of SRM data using freely available software. Important studies from the literature were highlighted to demonstrate the impact that SRM has had on the field of quantitative proteomics to date. The webinar concluded with a look to the future of quantitative proteomics and a discussion of the role SRM will play.