Unraveling O-glycosylation patterns with mucinases and mass spectrometry
Wednesday 6 November 2024
07:00 [PST] 10:00 [EST] 15:00 [GMT]
Alternative time zones
Leveraging mass spectrometry and mucinase enzymes to unravel O-glycosylation patterns, paving the way for immuno-oncology target discovery.
Aberrant O-glycosylation is gaining attention as a potential target in immuno-oncology. The presence of a single O-GalNAc residue (Tn antigen), considered a neoantigen, has emerged as a prime target. However, due to their low immunogenicity, targeting glycans remains a challenge. To address this, holistic approaches targeting Tn-carrying glycoproteins have been developed. Identifying potential proteins modified by the Tn antigen and the exact sites of modification remains difficult. This webinar will explore mass spectrometry approaches combined with recently described mucinase enzymes to elucidate O-glycosylation patterns on select recombinant O-glycoproteins, opening avenues for future O-glycoprotein target discovery and characterization.
What will you learn?
- The basics of O-glycosylation
- An overview of mass spectrometry approaches for O-glycopeptide characterization
- New approaches facilitating O-glycoprotein characterization
Who this may interest?
- Biomedical scientists in academia and industry
- Analytical scientists in academia and industry
Speaker
Tomislav Caval
Senior Scientist
Regeneron (NY, USA)
Tomislav Caval is an Analytical Chemist at Regeneron focusing on the characterization of biologics with an emphasis on mapping and understanding mucin type O-glycosylation. He obtained his PhD in Biomolecular Mass Spectrometry at Utrecht University (Netherlands) in 2019, under the supervision of Professor Albert Heck. During his PhD, he focused on developing new analytical approaches for mass spectrometric analysis of intact glycoproteins. In parallel, he developed methods for large-scale mapping of intact glycopeptides from cell lines, enabling insight into the landscape of mannose-6-phosphate-modified glycoproteins. He further honed his skills in the field of O-glycosylation by joining the lab of Professor Henrik Clausen, followed by a short postdoc at Stanford University (CA, USA) in the lab of Nobel laureate Professor Carolyn Bertozzi. Prior to joining Regeneron, he focused on studying the functional roles of glycosylation in human health and disease at InterVenn Biosciences (CA, USA) with the aim of developing improved liquid biopsies for early cancer detection.