Finding the needle in the haystack: A simple path to matrix removal and robust solid-phase extraction methods

In this seminar, we outlined several different approaches to sample clean-up using a variety of SPE tools. These approaches provide simple sample preparation solutions for a wide range of analytical needs, from routine analysis to highly selective analyte targeting and concentration.

Complex sample matrices create difficult challenges for scientists performing chromatography and mass spectrometry. Matrix interferences often cause matrix effects, inconsistencies in quantification and an overall lack of method robustness and reproducibility. These matrix components can also shorten column lifetime and lead to an increase in instrument downtime. Solid-Phase Extraction (SPE) is a very powerful tool that leads to more robust, reproducible analytical results by reducing matrix effects and chromatographic complexity while also providing the ability to increase analyte concentration. However, it can sometimes be challenging to know how and where to begin creating SPE methods. In this seminar, we will outline several different approaches to sample clean-up using a variety of SPE tools. These approaches provide simple sample preparation solutions for a wide range of analytical needs, from routine analysis to highly selective analyte targeting and concentration.

What will you learn?

• How to choose a Solid-Phase Extraction (SPE) sorbent appropriate for your needs
• SPE techniques to increase targeted analyte selectivity and sensitivity
• How to remove 95% of common matrix interferences with a 3 step SPE method
• Gain an understanding of the mechanisms involved with mixed-mode SPE

Who may this interest?

• Anyone working with bioanalytical sample matrices
• Scientists that need to isolate or concentrate compounds of interest away from complex sample matrices
• Anyone trying to reduce matrix effects and increase method robustness of complex samples

Speaker

Jon Danaceau
Principal Applications Chemist
Waters Corporation

Dr. Jon Danaceau is a Principal Applications Chemist in the Scientific Operations Group at Waters Corporation. He received a B.S. in biology at Allegheny College and a PhD in neuroscience at the University of Utah. He is currently focused on the development of clinical research and forensic toxicology methods employing sample preparation and LC-MS/MS.

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