Using Mitra sampling to support first-in-human pharmacokinetic evaluations for PF-07059013

5 Oct 2023

Journal articles MS (LC–MS/MS, HRMS, IMS)

C Lee K, X Wan K, Barricklow J, et al. | Bioanalysis, 15(17), 1083-1094, (2023) Keywords: • assay concordance • dried blood • LC–MS/MS • microsampling • Mitra • qualification Aim: A sensitive and selective method for the determination of PF-07059013 in dried blood collected by Mitra™ tips was developed and qualified from 50 to 50,000 ng/ml. Materials & methods: PF-07059013 is isolated from 10 μl of human dried blood by extraction with methanol and analyzed by HPLC–MS/MS. Results & conclusions: In addition to routine validation elements, impact of hematocrit and Mitra tip's lot-to-lot variation on assay accuracy were evaluated....