Tips and best practices to optimize your exosome labeling

Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, exosomes and their cargo are used as diagnostic biomarkers for cancer and other diseases. However, the isolation and detection of exosomes can be extremely challenging due to their small size (~30-200 nm in diameter, similar to most viruses). New methods and tools are constantly being developed but it can be difficult to know which to use.