Targeted full-scan LC-MS metabolomics: simultaneous quantitation of knowns and feature discovery provide the best of both worlds
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Abstract Mass spectrometry (MS) metabolomics, the direct measurement of the biochemical content of cells, tissues and biofluids by GC- or LC-MS, is a maturing discipline with the potential to quantitate and identify a wide range of biological small molecules. Measurement of metabolic state is orthogonal to genomics and proteomics, providing a unique and complementary view of biological state. Metabolomic analyses cover a broad and still expanding chemical space; analytical tools are needed for quantitation of known metabolites, and discovery and characterization of new compounds. Triple quadrupole (QqQ) mass spectrometers operating in multiple reaction monitoring (MRM) mode play a major role...
