Target occupancy biomarker assay development using a conformation-selective antibody against small-molecule-bound TNF

Hickford E S, O'Connell J, Maloney A, et al. | Bioanalysis, 15(6), 343-359, (2023) Keywords: • assay development • biomarker • ECLIA • ELISA • immunoassay • method development • small-molecule inhibitors • target engagement • target occupancy • TNF Background: An antibody specific to small-molecule inhibitor-bound TNF has enabled the development of target occupancy biomarker assays to support the development of novel treatments for autoimmune disorders. Materials & methods: ELISAs were developed for inhibitor-bound and total TNF to determine the percentage of TNF occupancy in samples from stimulated blood. Inhibitor-saturated samples allowed measurement of total and inhibitor-bound TNF in...