Systematic optimization of targeted and multiplexed MS-based screening workflows for protein biomarkers

Khanal N, Chen Z, Alelyunas Y, et al. | Bioanalysis, 14(6), 341-356, (2022) Keywords: • biomarkers • large molecule • LC–MS • matrix effects • method development • proteins • sample preparation Background: The capability of targeted MS-based methods to simultaneously measure multiple analytes with high selectivity and sensitivity greatly facilitates the discovery and quantitation of novel biomarkers. However, the complexity of biological samples is a major bottleneck that requires extensive sample preparation. Results: This paper reports a generic workflow to optimize surrogate peptide-based protein biomarker screening for seven human proteins in a multiplexed manner without the need for any...