Surrogate peptide selection and internal standardization for accurate quantification of endogenous proteins

Palshikar M, Jadav T, Rajput N, Kumar Sahu A & Sengupta P | Bioanalysis, 14(13), 949 - 961, (2022) Keywords: • endogenous proteins • high-resolution mass spectrometry • internal standardization • quantitative proteomics • surrogate peptide selection Relative quantification techniques have dominated the field of proteomics. However, biomarker discovery, mathematical model development and studies on transporter-mediated drug disposition still need absolute quantification of proteins. The quality of data of trace-level protein quantification is solely dependent on the specific selection of surrogate peptides. Selection of surrogate peptides has a major impact on the accuracy of the method. In this article, the...