Supercritical fluid chromatography for bioanalysis: practical and theoretical considerations

“...the development of robust apparatus and the improvement in the chromatographic systems knowledge seems to open a new period that should favor the use of SFC instead of many classical LC modes.” The definition of bioanalysis was well described by Rios et al. the separation of bioactive or endogenous compounds in biological matrice, as well as the respective performances of supercritical fluid chromatography (SFC) and HPLC in this field [1]. The use of SFC for bioanalysis could seem surprising due to the lack of water in the mobile phase, whereas most bioactive compounds are soluble in aqueous medium (plants and...