Quantitative HRMS

Welcome to the Bioanalysis Zone Spotlight on Quantitative HRMS!

HRMS has been available for a long time; originally it was applied in qualitative applications, however the introduction of spectrometers with improved mass resolution over wider mass ranges has allowed for the application of HRMS in quantitative workflows.

In the clinical setting, HRMS is increasingly being adopted due to its ease of operation, reasonable cost and maintenance. HRMS can also be combined with qualitative exploration of data in the small-molecule field.

In this Spotlight we will cover the advantages of HRMS and the remaining hurdles, such as; does the combination of qualitative and quantitative workflows result in less efficient bioanalytical assays?

Articles

HRMS using a Q-Exactive series mass spectrometer for regulated quantitative bioanalysis: how, when, and why to implement
A workflow for absolute quantitation of large therapeutic proteins in biological samples at intact level using LC-HRMS
High throughput analysis at microscale: performance of ionKey/MS with Xevo G2-XS QTof under rapid gradient conditions (Waters Corporation)
Outsource-ability of high-resolution MS-based DMPK assays
Modify on the fly: triple quad to high resolution in support of a dermal clinical study requiring an ultra low LLOQ
Application of high-resolution MS in the quantification of a therapeutic monoclonal antibody in human plasma
Integration of microfluidic LC with HRMS for the analysis of analytes in biofluids: past, present and future

Insights

Quantitative HRMS in the age of Biologics – a commentary on the recent HRMS survey
Quantitative HRMS Press Release – Exploring HRMS: the future is bright
Quantitative High Resolution Mass Spectrometry Coming of Age
Using HRMS in Bioanalysis – A conversation with Rand Jenkins and Patrick Bennett
Quantitative HRMS survey infographic
Alignment of Emerging Biomedical R&D Challenges with Tof-HRMS Capability and Versatility
Survey on Quantitative HRMS

Webinars

An evaluation of HRMS techniques on a Q-orbitrap vs. conventional SRM on a QQQ instrument…
Versatile Screening, Identification and Quantitation of Molecules with Q-Tof High Resolution Mass Spectrometer
Panel Discussion: The rise of Quantitative HRMS
Why compromise? The benefit of high resolution in MS and MS/MS for qualitative and quantitative analysis using a SCIEX TripleTOF System
Reaching New Heights in Targeted Quantitation with HRMS (Waters Corporation)
Evaluation of Ion Mobility and Targeted QTof acquisition modes for the quantitative analysis of peptides (Waters Corporation)

Interview

Using HRMS in Bioanalysis – A conversation with Rand Jenkins and Patrick Bennett

Resources

Protein quantitation workflows using the TripleTOF 6600: a case study for Rituximab (SCIEX)
Quantification of Large Oligonucleotides using High Resolution MS/MS on the TripleTOFTM 5600 System (SCIEX)
Highly Sensitive and Accurate Quantification of Isoprostane Oxidative Biomarkers using a High-Resolution Workflow (SCIEX)
Quantitation by High Resolution Mass Spectrometry: Case Study of TOF MS for the Quantitation of Allopurinol from Human Plasma (SCIEX)
Product: Xevo G2-XS QTof Mass Spectrometer (Waters Corporation)
Quantitation of high resolution MS data using UNIFI (Waters Corporation)
Xevo G2-XS QTof Mass Spectrometer: Extraordinary Possibilities for Your Everyday Analysis (Waters Corporation)