Overcoming Matrix Effects

Welcome to the Bioanalysis Zone Spotlight on Overcoming Matrix Effects!

Matrix effects are a major issue for the development and validation of bioanalytical methods. Bioanalysis Zone aims to advance the discussion in this crucial area with a series of articles, live events and interactive features.

Articles

Importance of matrix effects in LC-MS/MS bioanalysis
HILIC UHPLC–MS/MS for fast and sensitive bioanalysis: accounting for matrix effects in method development
Aging of biological matrices and its effect on bioanalytical method performance
Highly selective isolation and separation of 25-hydroxyvitamin D and 3-epi-25-hydroxyvitamin D metabolites from serum
Variability of the IS revealed ionization enhancement by bile acid in mouse plasma

Insights

Matrix effects – more than just phospholipids
Overcoming matrix effects: expectations set by guidelines
Overcoming Matrix Effects Spotlight survey infographic
Interview with Erin Chambers (Waters Corporation) on overcoming matrix effects in large molecule bioanalysis
Interview with Nico van de Merbel (PRA International) on the importance of matrix age
Interview with David Bell (Supelco) on matrix effects caused by phospholipids

Interviews

Interview with Erin Chambers (Waters Corporation) on overcoming matrix effects in large molecule bioanalysis
Interview with Nico van de Merbel (PRA International) on the importance of matrix age
Interview with David Bell (Supelco) on matrix effects caused by phospholipids

Resources

Clinical Testing web portal
Application Note: Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum (Sigma-Aldrich)
Application note: An Improved SPE-LC-MS/MS Method for the Quantification of Bradykinin in Human Plasma Using the ionKey/MS System (Waters Corporation)
Application Note: Robustness of ionKeyMS System in the Analysis of Pharmaceutical Compounds in Biological Fluids
Product: Hybrid SPE-Phospholipid Technology: Effective Removal of Phospholipids and Proteins for Accurate and Reproducible LC-MS Analyses
Application Note: Simultaneous Analysis of intact Human Insulin and Five Analogs in Human Plasma Using µElution SPE and a CORTECS UPLC Column
Application Note: Development of a Quantitative SPE LC-MS/MS Assay for Bradykinin in Human Plasma
Application Note: Chromatographic Reproducibility of the Novel ionKey/MS System
Application Note: Improving a High Sensitivity Assay for the Quantification of Teriparatide in Human Plasma Using the ionKey/MS System (Waters)
Application Note: Robustness of ionKeyMS System in the Analysis of Pharmaceutical Compounds in Biological Fluids