Hot topics in large molecule bioanalysis

Welcome to the Bioanalysis Zone Spotlight on ‘Hot topics in large molecule bioanalysis’!

Hot on the heels of our hugely popular ‘Overcoming matrix effects’ Spotlight, Bioanalysis Zone has released a new collection of articles, live events and interactive features.

This time the focus is on hot topics in large molecule bioanalysis. In recent years several important new challenges and vital advances have been sparking discussion in this ever-growing field. Covering a full spectrum of analytical techniques, we aim to reflect the growing interdisciplinary connections between ligand-binding assays and instrument-based analytics such as mass spectrometry.

Articles

Matrix effect in ligand-binding assay: the importance of evaluating emerging technologies
LC–MS/MS of large molecules in a regulated bioanalytical environment – which acceptance criteria to apply?
Development of a fast method for direct analysis of intact synthetic insulins in human plasma: the large peptide challenge
Systematic investigation of orthogonal SPE sample preparation for the LC–MS/MS bioanalysis of a monoclonal antibody after pellet digestion
Large molecule quantification: sensitivity and selectivity head-to-head comparison of triple quadrupole with Q-TOF
Laboratory automation of high-quality and efficient ligand-binding assays for biotherapeutic drug development
Insights into antibody-drug conjugates bioanalysis and biomeasures in discovery
LC-MS/MS of large molecules in a regulated bioanalytical environment – which acceptance criteria to apply?

Insights

Matrix effect in ligand-binding assay: the importance of evaluating emerging technologies
We are musicians in the same band: how LBA and LC-MS assays complement each other
Interview with Logan Umberger & Paul Rainville (Waters Corporation) on LC-MS for large molecule bioanalysis
Hot Topics in Large Molecule Bioanalysis Survey Infographic
Interview with Kelli Jonakin (AB SCIEX) on large molecule analysis by LC–MS
LC–MS/MS of large molecules in a regulated bioanalytical environment – which acceptance criteria to apply?
Interview with Robert Durham (Gyros) on bioanalysis of antibody-drug conjugates
LC-MS/MS of large molecules in a regulated bioanalytical environment – which acceptance criteria to apply?

Webinars

Panel Discussion: Hot Topics in Large Molecule Bioanalysis
Solutions for success: LC–MS in the changing landscape of large molecule bioanalysis
Bioanalysis of novel antibody constructs: approaches and challenges
Analyzing proteins with the latest innovation in reversed-phase column design
Protein and Peptide Therapeutics: Advanced tools for increasing bioanalytical sensitivity and specificity

Interviews

Interview with Suma Ramagiri (AB SCIEX) on quantification of large molecules
Interview with Logan Umberger & Paul Rainville (Waters Corporation) on LC-MS for large molecule bioanalysis
Interview with Kelli Jonakin (AB SCIEX) on large molecule analysis by LC–MS
Interview with Hillel Brandes (Sigma Aldrich) on advances in bioseparation of large molecules
Interview with Robert Durham (Gyros) on bioanalysis of antibody-drug conjugates

Resources

Poster: A sub-picogram quantification method for Desmopressin in plasma using the AB SCIEX Triple Quad™ 6500 System
Hot Topics in Large Molecule Bioanalysis Survey Infographic
Product: Gyrolab software modules (Gyros)
Product: Gyrolab CDs (Gyros)
Product: Triple QuadTM 6500 System (AB SCIEX)
Product: Rexxip buffers (Gyros)
Product: QTRAP® 6500 System (AB SCIEX)
Application note: Peptide/protein quantification with SCIEX separations
Application note: TripleTOF® 5600+ System (AB SCIEX)
Application note: Regulated Bioanalysis Application Notebook: Peptides (Waters)
Application note: An Improved SPE-LC-MS/MS Method for the Quantification of Bradykinin in Human Plasma Using the ionKey/MS System (Waters)
Application note: Advantages of Online 2D Chromatography for MRM Quantification of Therapeutic Monoclonal Antibodies in Serum (Waters)
Application note: Size Exclusion Analysis of Tween-containing IgG Formulations (Sigma-Aldrich)
Application note: BIOshell Fused-Core Columns for Ultra Efficient RPLC of Proteins and Peptides: Analysis of IgG fragments using a middle-down approach (Sigma-Aldrich)
Poster: Application of differential ion mobility mass spectrometry to peptide quantitation
Poster: Rapid Peptide and Protein Separations on BIOshell™ Fused-Core® U/HPLC Columns (Sigma-Aldrich)
Application note: Using Temperature to Improve Peak Shape of Hydrophobic Proteins in Reversed-Phase HPLC (Sigma-Aldrich)
Poster: Fit-for-Purpose Anti-Drug Antibody (ADA) Assay: Cut Point is More Important than Lower Limit of Quantitation (KCAS)
Application note: Development of an Anti-Drug Antibody Receptor Based Neutralizing Assay in Human Plasma (KCAS)
Poster: Development & Validation of a Direct Method for Quantification of DNA in Digested Tissue Samples (KCAS)
Application note: Modification of Commercial Kit Assay Conditions May Be Necessary for Increasing the Sensitivity of Biomarker Quantification: A Case Study (KCAS)
Poster: Use of Dilutional Linearity as a Screening Tool in Selection of a Commercial Kit for Biomarker Quantification: A case study (KCAS)
Application note: Miniaturizing immunoassays for improved performance (Gyros)
Poster: Fit-for-Purpose Validation of a Soluble CD14 ELISA in Human Plasma: Anomalous Long-Term Stability Pattern for Endogenous Biomarker (KCAS)
Poster: Faster Protein and Peptide Liquid Chromatography (FP2LC): IntroducingBIOshell™ Fused-Core® U/HPLC Columns (Sigma-Aldrich)
Application note: Advances in the bioanalysis of protein and peptide therapeutics through innovations in mass spectrometry (AB SCIEX)
Land O’Lakes: Analytical Considerations for Antibody Drug Conjugates