Single-drop microextraction in bioanalysis

Bioanalysis usually requires a preparation procedure for sample cleanup or preconcentration. Conventional sample preparation techniques are often time consuming and labor intensive. Among recent progress in sample preparation, single drop microextraction (SDME) is one of the most efficient techniques providing both sample cleanup and preconcentration capabilities. In SDME, analytes are extracted from a sample solution into an acceptor drop and the drop is introduced to subsequent analysis. Since the volume of the acceptor drop is 1–10 µl or less, the consumption of solvents can be minimized and the preconcentration effect is enhanced. In this review, the basic principles of two-phase...