Simultaneous measurement of mouse and human albumin in chimeric mice with humanized livers

Landry F, Shen H, Zhang Y et al. | Bioanalysis, 14(5), 267 - 278, (2022) Keywords: • albumin • chimera • hepatocytes • humanized • LC-MS/MS • liver • mice • plasma • PXB-mouse • quantification Background: The degree of human hepatocyte replacement in chimeric mice with humanized liver has previously been shown to correlate with human plasma albumin measurements. However, there are no reports that directly compare the remaining endogenous mouse albumin with the newly expressed human albumin following engraftment. To better understand the disposition of serum albumin in PXB-mice, we developed a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to simultaneously quantitate both human and mouse...