A simple, effective approach for rapid development of high-throughput and reliable LC–MS/MS bioanalytical assays

Quality and throughput are two primary goals for developing and optimizing LC–MS/MS bioanalytical methods. Various approaches and techniques have been evaluated and applied to LC–MS/MS bioanalysis to achieve these goals [1–11]. In addition to the throughput and quality of the bioanalytical assay itself, the speed of method development is also important. Depending on the stage of drug research and the scope of supported studies, the requirements (quality, throughput, sensitivity, etc.) of bioanalytical assays are quite different. Various method development strategies are needed to meet the requirements in different situations. In the discovery stage, bioanalysis is mostly used for exploratory purposes...