SFC-MS versus RPLC-MS for drug analysis in biological samples

“SFC–MS appears as a viable alternative to LC–MS for bioanalytical applications” The advantages of supercritical fluid chromatography (SFC) over LC have been widely described in the past and include enhanced kinetic performance, due to the lower fluid viscosity and better solute diffusion coefficient; lower consumption of organic solvents; highly efficient chiral separation in presence of a supercritical fluid; and improvement of productivity at the preparative scale [1–3]. However, all these benefits were restricted by the lack of robustness/reliability, limited sensitivity and poor quantitative performance of old-generation SFC systems. This is certainly why SFC has never been considered as a forefront...