Review on in vivo profiling of drug metabolites with LC-MS/MS in the past decade

Reddy GN, Laltanpuii C, Sonti R | Bioanalysis, 13(22), 1697–1722 (2021) Keywords: biotransformation • LC–MS • metabolite profiling • plasma • urine Metabolite profiling is an indispensable part of drug discovery and development, enabling a comprehensive understanding of the drug's metabolic behavior. Liquid chromatography-mass spectrometry facilitates metabolite profiling by reducing sample complexity and providing high sensitivity. This review discusses the in vivo metabolite profiling involving LC-MS/MS and the utilization of QTOF, QQQ mass analyzers with a particular emphasis on a mass filter. Further, a summary of sample extraction procedures in biological matrices such as plasma, urine, feces, serum and hair as in vivo samples are outlined. toward...