Redrawing Calibration Curves

I started in bioanalysis in 1969 when gas chromatography was ‘the technique’ for drug analysis. My interest in calibration mathematics and Monte Carlo techniques started in the early 1980’s when computer based chromatographic data systems appeared with differing regression mathematics, which were hotly debated by my then colleagues, especially with regard to weighting. Thanks to a brilliant maths teacher, I began to understand weighted regressions, teach others, and investigate the limitations of obtaining precise calibration curves with small data populations of calibrators. Around this time I also became interested in non-linear behaviour of assays and developed the regression for y...