Quantifying the role of oxygen gradients in tissue and tumor-like 3D cultures
Monday 29 January 2018 at 07:00 [PST] 10:00 [EST] 15:00 [GMT]
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Matthew Lockett has been named the winner of the 2017 New Investigator Award! Register for our webinar and find out more about Matthew’s research:
Despite the pivotal role oxygen plays in tissue and tumor biology, there are a limited number of in vitro assays able to quantify cellular responses in well-defined oxygen gradients. We are developing a 3D culture platform, which utilizes paper-based scaffolds, to prepare tissue- or organ-like structures. We are able to engineer extracellular environments with oxygen or nutrient gradients of defined steepness. In this talk, I will highlight the paper-based culture platform ability to address three long-standing questions in oxygen biology and as a screening platform to evaluate new drugs and potential toxicants.
What will you learn?
- Current experimental challenges of studying cellular responses in oxygen gradient
- 3D culture platform development and validation
- Sensors capable of mapping gradient formation and maintenance in 3D cultures
- Analytical methods for evaluating cellular responses at the gene and protein level in oxygen gradients
Who may this interest?
- Analytical biochemists, interested in oxygen mediated protein expression and regulation
- Biomedical engineers and chemists developing microfluidic technologies
- Developers of paper-based diagnostics
- Individuals developing high-throughput screening platforms for cellular responses to drugs, small molecule modulators, or biologics
- Individuals developing animal-free means of testing drugs and therapies
