Protein quantification using LC–MS: can it make a difference?

 “LC–MS can certainly add value for protein quantification, in particular because of its reliable nature and the resulting improved comparability of results between laboratories.” Over the past few years, there has been a remarkable increase in the interest to apply LC–MS-based techniques for the quantification of proteins and other large molecules in complex biological samples. While LC–MS has matured into the golden standard for small-molecule bioanalysis, the field of protein quantification has been dominated by ligand-binding assays (LBAs) for several decades, mainly because of their superior sensitivity and their compatibility with intact large molecules. With the introduction of increasingly sensitive...