Merck: Supelco HybridSPE®-PLus 96-well plates and cartridges

Profile: Remove both precipitated proteins and phospholipids from biological samples in just a few simple steps for accurate and reproducible LC-MS analyses.

Rapid biological sample prep method method for more accurate and reproducible data

Eliminates ion suppression, column back pressure, and the need for gradient column cleanups by selectively removing phospholipids and proteins via a simple pass through method (no preconditioning required)
Prepares biological samples for downstream LC/MS or LC/MS/MS analysis
Selective retention mechanism works even with very hydrophobic analytes (such as vitamins), that are lost when using competitive products
High throughput 96-well or individual cartridge formats available

HybridSPE-Phospholipid Technology

HybridSPE combines the simplicity of protein precipitation with the selectivity of Solid Phase Extraction (SPE) for the targeted removal of phospholipids and proteins from biological samples. The technology utilizes a zirconia-coated particle, and exhibits selective affinity towards phospholipids while remaining non-selective towards a range of basic, neutral and acidic compounds. The phospholipid retention mechanism is based on highly selective Lewis acid-base interaction between the proprietary zirconia ions (functionally bonded to the HybridSPE stationary phase) and the phosphate moiety consistent with all phospholipids.

Learn more about the HybridSPE:

For general information on the HybridSPE technology please click here
For a detailed brochure on HybridSPE Technology Bridging the Gap between Simplicity and Selectivity in Pharma Bioanalytical Sample Prep please click here.
To find out more about how the technology works please click here
To download HybridSPE Method Development: Recovery Optimization please click here
To download a troubleshooting guide please click hereand for Frequently asked Questions please click here

Download articles about HybridSPE by clicking on the links below:

Vitamin D epi-Metabolites Separated with HybridSPE: To download full article click here
Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma: To download full article, click here
Enrichment of Phospholipids in Biological Samples Using HybridSPE: To download full article click here
Ion-Suppression & Phospholipid Contamination : To download full article click here
Improve LC-MS Run Time/Performance & Reduce Risk of Ion-Suppression : To download full article click here
Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples: To download full article, click here

For more information please click here