Bio-Rad: Recombinant fully human isotype controls
Use fully-human recombinant monoclonal antibodies as negative controls in antibody drug discovery and development, and in vitro diagnostic assays. Negative controls differentiate the non-specific background signals initiated by the antibody Fc domain or other cellular protein interactions, from the target-specific antibody signal. Bio-Rad’s recombinant antibodies are expressed in a mammalian cell line, ensuring continuity of supply and consistent quality from batch to batch, and overcome the challenges of sourcing control immunoglobulins from human sera.
- IgG isotypes 1, 2, 3 and 4, IgA, IgE and IgM
- Sequence of heavy and light chain known, full allotype information available
- Specific for Green Fluorescent Protein (GFP), not found in mammalian cells
- IgG4 available as wildtype and point mutant S228P
- IgG1 available in allotypes G1m3 and G1m17,1
- Preservative free and low endotoxin format on request, for in vivo work