Merck: Ascentis® Express Products: Columns for starting a new method and lipid analysis

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C18 HPLC Column

 

Ascentis® Express C18 is the first choice for starting a new method. Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles at lower system pressure. With identical conditions, the Ascentis Express C18 column performs more than 4X faster than a standard C18 column, for most applications. The combination of high efficiency and lower operating pressure benefits UHPLC (or other ultra high pressure system) users, as well as conventional HPLC users. The increased efficiency of Ascentis Express columns enables you to use shorter columns and reduce solvent

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To read articles about the C18 column click the links below:

  • Molecularly Imprinted Polymer SPE- Acid NSAIDs from Muscle: To download the full article click here
  • Fast HPLC Using Fused-Core Particle Technology for Rapid Screening of Pharmaceutical Compounds: To download the full article click here
  • Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3l: To download the full article click here
  • Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS: To download the full article click here
  • Serum Lipidomics Profiling using an Ascentis Express Fused-Core C18 Column: To download the full article click here
  • Accelerated Bioanalytical LC-MS-MS | Ascentis® Express: To download the full article click here

F5 HPLC Columns

The pentafluorophenylpropyl (PFP) stationary phase of Ascentis® Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

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To read articles about F5 HPLC Columns click on the links below:

  • Fast and Accurate LC-MS Analysis of Vitamin D Metabolites Using Ascentis® Express F5 HPLC Columns: To download the full article click here
  • Alternative Retention and Selectivity Using Fluorinated Stationary Phases: To download the full article click here