Prediction of frozen virus stability based on degradation mechanisms, real-time data and modeling

Homan Y, Rosenbloom D, Wong S, et al. | Bioanalysis, 14(17), 1177-1190, (2022) Keywords: • clinical assay • cryopreservation • mathematical modeling • prediction • virus preservation • virus stability Aim: Critical virus reagents in regulated bioanalytical assays require stability monitoring. Although stability at ultra-low frozen temperatures is generally assumed, published data are limited and real-time studies are time consuming. Materials & methods: The authors reviewed literature data, typical mechanisms of molecular degradation, glass transition temperatures of commonly used buffers and available real-time storage data to model frozen virus reagent stability. Results: Storage at ultra-low temperatures below the glass transition...