Neuro biomarker levels measured with high-sensitivity digital ELISA differ between serum and plasma

In this Research Article, published by Bioanalysis, investigators from Case Western Reserve University (OH, USA) investigate whether different preanalytical blood processing techniques influence results of digital enzyme-linked immunosorbent assay (ELISA)-based assays for blood biomarkers. Keywords: brain injury, methods, neurodegeneration, NFL, SiMoA, single molecule array, techniques Aim: Digital ELISA-based assays for blood biomarkers of neurological disease are on the verge of clinical use. Here, we aimed to determine whether different preanalytical blood processing techniques influence results. Materials & methods: Concentrations of neurofilament light chain (NfL), Tau and amyloid beta (Aβ) were measured in human plasma and serum specimens using digital ELISA and compared between blood products. Measured levels of NfL were highly equivalant...