Method development workflow for quantifying protein biomarkers by hybrid LC–MS/MS

Agrawal K , Shi Y , Del Rosario A & Jian W | Bioanalysis, 14(14), 985 - 1004, (2022) Keywords: • biomarker • endogenous protein • fit-for-purpose • hybrid LC–MS/MS • immunoprecipitation • method development • parallelism Background: Industry-standard guidance on method development and validation of hybrid LC–MS/MS assays for protein biomarkers, particularly on evaluation of parallelism, is lacking. Methods: Using a protein endogenous to humans and mice as a model analyte, a quantitative hybrid LC–MS/MS workflow was developed using a surrogate matrix approach with a recombinant form of the protein as the calibrant. Results: The developed workflow identified a...