Liquid chromatographic methods in the determination of inosine monophosphate dehydrogenase enzyme activity: a review

Perdalkar S, Yarlagadda D, Ganesh Pai C, Shetty S & Bhat K | Bioanalysis, 14(22), 1453 - 1470, (2023) Keywords: • inosine-5′-monophosphate dehydrogenase • liquid chromatography • mass spectrometry • mycophenolic acid • pharmacodynamic biomarker • thiopurine Inosine monophosphate dehydrogenase (IMPDH) is a crucial enzyme involved in the de novo synthesis of purine nucleotides. IMPDH activity is used to evaluate the pharmacodynamics/pharmacokinetics of immunosuppressant drugs such as mycophenolic acid and thiopurines. These drugs are often used to prevent organ transplant rejection and as steroid-sparing agents in autoinflammatory diseases such as inflammatory bowel disease and rheumatoid arthritis. Numerous analytical techniques have...