Ligand-binding assays: old challenges, new solutions

“I would imagine within the next 5 to 10 years we will see immunoassays become as robust as chromatographic methods.” In a very real sense, we would not have immunoassays at all if it were not for the occurrence of insulin resistance in diabetic patients treated with porcine insulin. In the case of Solomon Berson and Rosalyn Yallow and the invention of radioimmunoassay (RIA), first, this was dependent upon the availability of a pure reference standard, in this case crystallized insulin. Second, was the discovery that you can treat diabetic patients with insulin extracted from porcine pancreas and the ability...