LC–MS/MS technology for large and small molecule quantitation: key challenges in drug discovery

In this commentary from Shashyendra Singh Gautam (Biocon Bristol-Myers Squibb R & D Center, Karnataka, India), explore the use of LC–MS/MS technology for large and small molecule quantitation, key challenges associated with the technology and the application of LC–MS/MS in drug discovery bioanalysis. LC–MS/MS technology is one of the most powerful analytical tools available and is pivotal in answering questions in drug discovery and the development of both small and large molecules. The instrument’s versatility, robustness, selectivity and sensitivity make it a gold standard for small molecule bioanalysis, and it is making firm strides in achieving the same with large...