LC–MS/MS method for simultaneous estimation of raloxifene, cladrin in rat plasma: application in pharmacokinetic studies

Chauhan D, Dadge S, Yadav P, et al. | Bioanalysis, 16(03), 141-153, (2024) Keywords: • bioanalytical method validation • co-estimation • LC–MS/MS • pharmacokinetics • raloxifene and cladrin • simultaneous identification Aim: A newer LC–MS/MS method was developed and validated for the simultaneous quantification of raloxifene (RL) and cladrin (CL). Methodology: Both drugs were resolved in RP-18 (4.6 × 50 mm, 5 μ) Xbridge Shield column using acetonitrile and 0.1% aqueous solution of formic acid (FA) (70:30% v/v) as mobile phase by using biological matrices in female Sprague–Dawley rats using–MS/MS. Results: The developed method was found to be linear over the concentration ranges of 1–600 ng/ml, and lower limit of quantification was 1 ng/ml for RL and CL, respectively. Pharmacokinetic results of RL+CL showed...