Large molecule bioanalysis by LC–MS: beyond simply quantifying

 Merbel N, Olaleye O, Spanov B & Bischoff R | Bioanalysis, 14(7), 397-400, (2022) Keywords: • biomarkers • biopharmaceuticals • biotransformation • heterogeneity • LC–MS • proteins During the last decade, the quantitative bioanalysis of biopharmaceuticals and large molecule biomarkers by liquid chromatography–mass spectrometry (LC–MS) has been an important research topic in our group. Since we, and many others, started asking ourselves whether LC–MS could make a difference for targeted protein quantification [1], the world has seen important developments in the technology. LC–MS can now definitely be regarded as an established platform for protein bioanalysis, next to the traditional ligand-binding...