Introducing dendritic cell antibody internalization as an immunogenicity risk assessment tool

Melendez R, Ordonia B, Guerrero J, et al. | Bioanalysis, 14(10), 703 - 713, (2022) Keywords: • anti-drug antibodies • APCs • biotherapeutics • dendritic cells • immunogenicity • internalization index Aim: Immunogenicity risk assessment assays are powerful tools that assess the relative immunogenicity of potential biotherapeutics. We detail here the development of a novel assay that measures the degree of antibody internalization by antigen-presenting cells as a predictor of immunogenicity. Results & methodology: The assay uses the fluorescence signal from the antibody bound to the outside of the cell as well as inside the cell to determine internalization. To...