In vitro immunogenicity prediction: bridging between innate and adaptive immunity

Cohen S, Chung S | Bioanalysis, Epub Ahead of Print (2021) Keywords: antidrug antibody • antigen internalization • DC activation • DC maturation • DC:T cell assay • HLA-II • immunogenicity • in vitro T-cell assay • MAPPs • T-cell proliferation Development of antidrug antibodies (ADAs) is an undesirable potential outcome of administration of biotherapeutics and involves the innate and adaptive immune systems. ADAs can have detrimental clinical consequences: they can reduce biotherapeutic efficacy or produce adverse events. Because animal models are considered poor predictors of immunogenicity in humans, in vitro assays with human innate and adaptive immune cells are commonly used alternatives that can reveal...