In the Zone: phosphorylated biomarkers

An integral part of the drug development process is the proper identification of drug action and mechanism. One method to understand the pharmacodynamic effects of a drug on specific diseased or rare cell populations involves detecting signaling pathway activation and key phosphorylated members of the pathway chain.  Proteins phosphorylated by a kinase with a phosphate group from high energy molecules such as ADP or ATP can act as such biomarkers for the progression of various leukemias. In this feature, we will further explore the role of phosphorylated biomarkers as an indication of drug efficacy during the drug development process.

INFOGRAPHIC

Infographic: the use and applications of Phosphoflow – In this infographic we look at Phosphoflow, a flow cytometry-based technique with many potential uses in support of drug development.

PODCAST

An interview with Teona Roschupkina and Richard Hughes: phosphorylated biomarkers – In this podcast interview, Teona Roschupkina (Principle Scientist-Flow Cytometry, LGC; Fordham, UK) and Richard Hughes (Associate Scientific Director, LGC; Fordham, UK) discuss the role of phosphorylated biomarkers as an indication of drug efficacy during the drug development process.

RESOURCES

Flow cytometry in cell-based pharmacokinetics or cellular kinetics in adoptive cell therapy

Two-step protocol for preparing adherent cells for high-throughput flow cytometry

Flow cytometry: a flexible tool for biomarker research

In association with:

[Video edit by Andrew McKinnon]