Highly specific and sensitive immunoassay for the measurement of prostaglandin E2 in biological fluids
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This is article is available as part of our Focus on China, to view all other articles available as part of this feature click here. Background: Lack of specificity of anti-PGE2 antibodies is a long-standing problem. Given quite a few analogs and low PGE2 content in biological fluids, it is quite important to simultaneously meet the demands of high specificity and sensitivity. Results: Highly specific anti-PGE2 antibodies were obtained by combined use of cationic carrier protein and Mannich reaction. The cross-reactivity values of the resultant polyclonal and monoclonal antibodies against eight analogs were <14 and <5%, respectively. Furthermore, we established...
