ELISA array-based immunoassay for multiplex detection of osteoporosis-relevant biomarkers

Li Y, Ni J, Gu J, Wang Y, Xiao L & Sun L | Bioanalysis, 15(2), 63-70, (2023) Keywords: • bone turnover markers • ELISA array • N-terminal propeptide of type I collagen • osteocalcin • osteoporosis biomarkers • parathyroid hormone Aim: This study aimed to develop an ELISA array-based immunoassay for the simultaneous detection of osteoporosis-relevant biomarkers. Methods: ELISA array was constructed and its performance was evaluated using commercial antigens. The validity was further confirmed using traditional ELISA. Results: The constructed ELISA array showed good repeatability. The linear ranges and limits of detection for the four markers mentioned above...