Development and validation of HILIC–ESI/MS/MS methods for simultaneous quantitation of several antipsychotics in human plasma and blood

Background: Knowledge of antipsychotic drug levels at point of care (POC) may significantly aid therapeutic decision-making. To support the development of future POC devices and to validate the use of fingerstick capillary blood sampling, two robust hydrophilic interaction LC–ESI/MS/MS methods were developed and validated. Two PK studies were completed evaluating the correlation between fingerstick blood and plasma concentrations with corresponding venous blood and plasma concentrations for several commonly prescribed atypical antipsychotics and selected metabolites. Sensitive and reliable LC–MS/MS bioanalytical assays were developed to support these studies. Results: Three methods, requiring only 25-μl matrix volumes, were developed using supported liquid extraction...