Development and validation of carbodiimide-activated ELISA plate for quantifying IgG levels against Streptococcus pneumoniae capsular polysaccharides

Ramakrishnan SM, Mavupadi AM, Govindan V, Nagaraj G & Ravikumar KL | Bioanalysis, 16(13), 641–650, (2024) Keywords: • carbodiimide activation • IgG antibodies • microtiter plate • polysaccharide antigens • Streptococcus pneumoniae • validation Background: Conventional microtiter plates lack the surface strength needed for effective binding of pneumococcal polysaccharide antigens. This study tackles the limitation by altering the surface of polystyrene plates through carbodiimide activation under acidic pH conditions. Method: The microtiter plates were activated with carbodiimide coupling agents, N,N′-Dicyclohexylcarbodiimide (DCC) and N-Hydroxysuccinimide (NHS). They were subsequently coated with 13 pneumococcal antigens at a concentration of 5 μg/ml with a...