Determination of cortisol in hair using liquid chromatography-tandem mass spectrometry: a short review

Soares DRD, Antunes MV, Linden R | Bioanalysis, doi: 10.4155/bio-2021-0101 (2021) (Epub ahead of print) Keywords: cortisol • hair • LC–MS/MS • liquid chromatography • mass spectrometry Cortisol is considered a particularly relevant biomarker in the context of stress evaluation. This study aims to review of the available literature on the determination of cortisol in hair using LC-MS/MS. Currently, there is no standardized procedure for the measurement of cortisol concentrations in hair, and different sample preparation, chromatographic separation and mass spectrometric detection conditions were described. Simple methanolic extraction, reversed-phase separation and MRM detection in negative ion mode are the most common employed...