Critical reagents in flow cytometry, instrumentation and application in drug discovery development

V N Kakkanaiah, K Matys & P Bennett | Bioanalysis, 13(10), 841-846 (2021) Keywords: • critical reagents • flow cytometry • LBA Flow cytometry and ligand-binding assays (LBA) have been used in all stages of drug development, from early discovery to preclinical and clinical studies. Flow cytometry is a powerful tool to analyze multiple characteristics of a single cell/particle simultaneously by optical means using laser light and the multiparametric analysis is performed on individual cells at a very high rate. Multiple cell populations can be characterized using a combination of reagents against cell surface and intracellular markers in any single...