Critical considerations of matrix selection in LC–MS bioanalysis for toxicokinetic and pharmacokinetic assessment in drug development

Fu Y, Li W & Flarakos J | Bioanalysis, 13(8), 605-608, (2021) Keywords: • bioanalysis • blood/plasma distribution • blood stability • matrix selection • plasma • protein binding • stability • whole blood In drug development, plasma rather than whole blood has been the primary biological matrix for the toxicokinetic (TK) or pharmacokinetic (PK) assessment of drug candidates [1,2]. The preference in using plasma over blood in these assessments is not only owing to the homogeneous nature of plasma, but also, more importantly, due to the fact that plasma concentration is generally more relevant than blood to the effect of the drug [3]. In contrast, blood is more suitable than plasma for monitoring...