Challenges of implementing ultrahigh-throughput mass spectrometry in absorption, distribution, metabolism and excretion bioanalysis

Wang Y, Pu F | Bioanalysis, 15(20), 1217-1270, (2023) Keywords: • ADME • bioanalysis • high-throughput screening • HTS • non-probe-based assays • probe-based assays • ultrahigh-throughput mass spectrometry Ultrahigh-throughput mass spectrometry (uHT–MS) refers to analytical tools capable of acquiring mass spectra of a sample within the duration of 1 s or less. This level of sampling speed for MS is enabled by the development of nonconventional sample introduction pathways that completely bypass liquid chromatography (LC) separation and sample directly from minimally prepared samples. Consequently, it leads to a significant enhancement in sample throughput within the designated time frame. Examples...