Can MS fully exploit the benefits of fast chromatography?

“Using data-dependant or -independent acquisition workflows, modern mass spectrometric instrumentation can easily generate large volumes of data. The challenge is now in the data processing to extract the relevant information to address relevant biological questions.” Over the last two decades, LC coupled to MS detection (LC–MS) has become a reference analytical method for qualitative and quantitative analysis of proteomics, food science, pharmaceuticals, toxicology and environmental samples. With regards to LC, most of the applications use reverse phase, normal phase or hydrophilic interaction LC with various configurations from nano-LC (100 µm internal diameter [i.d.] columns) to conventional bore LC (4.6 mm...