Assay dynamic range for DBS: battles on two fronts

“Even if the overall extraction recovery from the card and the subsequent liquid processing is similar to the recovery of a plasma assay, bioanalysts need to have a wide selection of techniques to improve sensitivity by five- to 20-fold for low-dosing clinical studies and assays with metabolites.” The use of dried blood spots (DBS) to generate toxicokinetic and pharmacokinetic data has been of great interest in the pharmaceutical industry recently. One of the main advantages is microscale sampling, which aligns perfectly with the 3Rs principles (replacement, refinement and reduction) for animal use in research. The DBS technique offers benefits in...