Approaches to improve drug tolerance and target tolerance in the assessment of neutralizing anti-drug antibodies

In this Research Article, published by Bioanalysis, investigators from Janssen Research & Development LLC and Thomas Jefferson University (both PA, USA) describe potential approaches for enabling the development of reliable neutralizing anti-drug antibody (NAb) assays that overcome drug and drug target interference for more precise and sensitive NAb assessment. Keywords: acid dissociation, affinity capture elution, biotherapeutic, drug tolerance, drug target tolerance, neutralizing antibody Aim: Neutralizing anti-drug antibody (NAb) assays are inherently prone to the interference from drug and its soluble target, potentially resulting in erroneous results. An effective approach to improve drug tolerance of an NAb assay is pretreatment of samples with acid to dissociate immune complexes of NAb...