Application note: BIOshell Fused-Core Columns for Ultra Efficient RPLC of Proteins and Peptides: Analysis of IgG fragments using a middle-down approach (Sigma-Aldrich)

Columns packed with fully porous particles have long been considered ‘normal science’ for HPLC practitioners. Since 2006, Fused-Core® or core-shell columns have caused a paradigm shift in the minds of liquid chromatographers who analyze small molecules (1). The benefits of porous-shell columns are now also available to scientists who are tasked to advance our knowledge about peptides, proteins and other biopolymers.