Application note: Advantages of Online 2D Chromatography for MRM Quantification of Therapeutic Monoclonal Antibodies in Serum (Waters)
Virus protection. Vaccine search. Antibodies and viral infection. Immune defense of the body. Attack on antigens 3D illustration
In this application note, we demonstrate that a two-dimensional chromatographic assay (2D LC or 2D UPLC) for mulitple reaction monitoring (MRM) quantification in protein bioanalysis can provide up to a three-fold increase in sensitivity compared to one-dimensional LC/MRM. Additionally, high pH/low pH RP/RP separations can significantly reduce analyte suppression.
