Application of DMS in boosting sensitivity for protein digest LC–MS/MS workflow

Introduction

• Low sensitivity methods in protein LC–MS bioanalysis commonly utilise a ‘bottom-up’
  approach. However, lack of selectivity can limit the sensitivity achievable
• Often specific protein level clean-up is not an option, due to cost, time or unavailability of
  suitable reagents, leading to a complex mixture of peptides generated for analysis
• With only 20 naturally occurring amino acid building blocks, isobaric interference within
  specific monitored SRM transitions is inevitable
• Instrumental techniques offer a useful means of adding selectivity, such as high resolution
  mass spectrometry, multi-dimensional chromatography or ion mobility
• Differential Ion Mobility (DMS) is one such subset, offering fast separations through a user
  removable short planar mobility cell situated in in the ion source region
• LLOQ samples from two validated protein digest assays

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